町田　千代子　MACHIDA Chiyoko

プロフィール

職名	教授
所属	応用生物学部 応用生物化学科 大学院 応用生物学研究科 応用生物学専攻
最終学歴	千葉大学理学部
学位	農学博士（東京大学）
所属学会・役職	日本分子生物学会 日本遺伝学会 日本植物学会 日本植物生理学会 日本RNA学会会員 バイオインダストリー協会会員
専門分野	植物分子発生学
研究テーマ	植物の発生分化の分子メカニズムの研究
授業科目	分子生物学、遺伝子工学、分子生物学実験、細胞生物学特論
共同研究キーワード	ケミカルジェネティクス

著書および訳書

岩川秀和・町田千代子　左右相対称で扁平な葉がつくられる分子メカニズムを探る「目で見るバイオ」バイオインダストリー　Vol.65 No.4 8-9, 2007

町田千代子・岩川秀和・小島晶子 左右相対称で扁平な葉がつくられる分子メカニズムを探る「解説」バイオインダストリー Vol.65 No.4 16-20, 2007

岩川秀和・町田泰則・町田千代子　マップベースクローニング「改訂3版モデル植物の実験プロトコール」（監修　岡田清孝他） 細胞工学別冊植物細胞工学シリーズ 21, 116-120, 2005

葉の形造りにおける左右相対称性と扁平性　町田千代子・上野直人・小島晶子・町田泰則　学術月報　58, 6-11, 2005

小島晶子・町田千代子　植物形態形成における体軸決定の仕組み「発生システムのダイナミクス」（編集：上野直人・八杉貞雄・野地澄晴）「蛋白質・核酸・酵素」増刊号 50, 724-730, 2005

田中博和・町田千代子・町田泰則　トルイジンブルー法―シロイヌナズナの表皮の異常を検出する 「改訂3版モデル植物の実験プロトコール」（監修　岡田清孝他）細胞工学別冊植物細胞工学シリーズ 21, 70-71, 2005

Machida C., Iwakawa, H., Ueno, Y., Semiarti, E., Tsukaya, H., Hasebe, M．Kojima, S. and Machida, Y.　"Morphogenesis and Pattern Formation in Biological Systems - Experiments and Models -" edited by T. Sekimura, S. Noji, N. Ueno, and P.K. Maini. Published by Springer-Verlag Tokyo, pp177-187, 2003

町田千代子 植物の形造り：葉の組み立て．明日を拓く植物科学．光りエネルギーを生物エネルギーにかえる植物の設計図を読む．第16回「大学と科学」公開シンポジウム講演収録集．クバプロ 38-47, 2002

町田千代子・上野宜久・町田泰則 葉の形成 植物ゲノム機能のダイナミズム：転写因子による発現制御　（監修：岩渕雅樹、篠崎一雄 ）シュプリンガー・フェアラーク東京 pp46-56, 2002

上野宜久・町田千代子・町田泰則. 葉の発生分化における左右相称性と扁平性の制御機構．植物の形づくり：遺伝子から見た分子メカニズム（編集：岡田清孝・町田泰則・島本功・福田裕穂・中村研三）蛋白質核酸酵素2002年9月号増刊：1570-1575, 2002

町田千代子・田中博一・岩川秀和、町田泰則 トランスポゾンを利用した遺伝子クローニング ブレインテクノニュース78, pp18-23, 2000

Machida C., Onouchi H., Endang S., Ishikawa T., Machida Y.：Use of the R-RS site-specific recombination system in plants.　Plant Molecular Biology Manual (ed. by Gelvin, S.B.) published by Kluwer Academic Publishers (Netherland) N2, 1-23 (2000)

塚谷裕一、町田千代子 双子葉植物における葉の形成 植物細胞工学シリーズ12 「植物の形をきめる分子機構」（監修：岡田清孝、町田泰則 、松岡信 ）秀潤社 pp103-116 (2000)

大坪栄一・W. Doroszkiewicz・D. Davison・K. Nyman・大坪久子・町田千代子・町田泰則挿入因子 IS1 の構造と機能の発現 組換え DNA 実験 II、高木康敬編、共立出版、103-119 (1983)

学術論文、評論

主な発表

Matsumura, Y., Iwakawa, H., Machida, Y., Machida, C. 2009. Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana and functional and molecular comparisons between AS2 and other family members. Plant J. on line

Takahashi H., Iwakawa H., Nakao S., Ojio T., Morishita R., Morikawa S., Machida Y., Machida C., Kobayashi T.: Knowledge-based Fuzzy Adaptive Resonance Theory and its Application to the Analysis of Gene Expression in Plants. Journal of Bioscience and Bioengneering 106(6), 587-593, 2008

Yang JY., Iwasaki M., Machida C., Machida Y., Zhou X., Chua NH.: betaC1,the pathogenicity factor of TYLCCNV, interacts with AS1 to alter leaf development and suppress selective jasmonic acid responses. Genes & Development 22, 2564-2577, 2008

Ishikawa T., Machida C., Yoshioka Y., Ueda T., Nakano A., Machida Y.: EMBRYO YELLOW gene, encoding a subunit of the conserved oligomeric Golgicomplex, is required for appropriate cell expansion and meristem organization in Arabidopsis thaliana. Genes to Cells 13, 521-535, 2008

Kitakura S., Terakura S., Yoshioka Y., Machida C., Machida Y.: Interaction between Agrobacterium tumefaciens oncoprotein 6b and a tobacco nucleolar protein that is homologous to TNP1 encoded by a transposableelement of Antirrhinum majus. J. Plant Res. 121, 425-433, 2008

Iwakawa H., Iwasaki M., Kojima S., UenoY., Soma T., Tanaka H., Semiarti E., Machida Y. and Machida C. : Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves. Plant J. 51, 173-184, 2007

Tanaka H., Watanabe M., Sasabe M., Hiroe T., Tanaka T., Tsukaya H., Machida C. and Machida Y. : Novel receptor-like kinase ABNORMAL LEAF SHAPE2 controls embryonic patterning and post-embryonic development of shoot organs through epidermal differentiation in Arabidopsis. Development 134, 1643-1652, 2007

Semiarti E., Indrianto A., Purwantoro A., Isminingsih S., Suseno N., Ishikawa T., Yoshioka Y., Machida Y. and Machida C. : Agrobacterium-mediated transformation of the wild orchid species . Phalaenopsis amabilis, Plant Biotechnology 24, 265-272, 2007

Terakura S., Ueno Y., Tagami H., Kitakura S., Machida C., Wabiko H., Aiba H., Otten L., Tsukagoshi H., Nakamura K., Machida Y. : An oncoprotein from the plant pathogen Agrobacterium has histone-chaperone-like activity. Plant Cell, 19,2855-2865, 2007

Ueno Y., Ishikawa T., Watanabe K., Terakura S., Iwakawa H., Okada K., Machida C. and Machida Y. : Histone Deacetylases and ASYMMETRIC LEAVES2 Are Involved in the Establishment of Polarity in Leaves of Arabidopsis. Plant Cell 19, 445-57, 2007

Terakura S., Kitakura S., Ishikawa M., Ueno Y., Fujita T., Machida C., Wabiko H. and Machida Y. : Oncogene 6b from Agrobacterium tumefaciens induces abaxial cell division at late stages of leaf development and modifies vascular development in petioles. Plant & Cell Physiology 47, 664-672, 2006

Watanabe M., Tanaka H., Machida C., Watanabe D, Machida Y. : The ACR4 receptor-like kinase is required for surface formation of epidermis-related tissues in Arabidopsis thaliana. Plant J. 39, 298-308, 2004

Kitakura S., Ueno Y., Terakura S., Machida C., and Machida Y. : Mechanism of proliferation of plant cells that is induced by oncogene 6b from Agrobacterium tumefaciens: interaction of the 6b protein with a putative transcription factor in tobacco cells. Endocytobiosis and Cell Research 15, 179-180, 2004

Tanaka H., Ishikawa M., Kitamura S., Takahashi Y., Soyano T,, Machida C. and Machida Y. : The AtNACK1/HINKEL and STUD/TETRASPORE/AtNACK2 genes, which encode functionally redundant kinesins, are essential for cytokinesis in Arabidopsis. Genes to Cells 9, 1199-1211, 2004

Tanaka T., Tanaka H., Watanabe M., Machida C., Machida Y. : A new method for rapid visualization of defects in leaf cuticle reveals five intrinsic patterns of surface defects in Arabidopsis. Plant J. 37, 139-146, 2004

Machida C., Ueno Y.and Machida Y. : Function of the ASYMMETRIC LEAVES1 and ASYMMETRIC LEAVES2 genes in leaf development of Arabidopsis. Plant Morphology 15, 30-39, 2003

Ishikawa T., Machida C., Yoshioka Y., Kitano H., and Machida Y. : The GLOBULAR ARREST1 gene, which is involved in the biosynthesis of folates, is essential for embryogenesis in Arabidopsis thaliana. The Plant Journal 33, 235-244, 2003

Machida C., Iwakawa, H., Ueno, Y., Semiarti, E., Tsukaya, H., Hasebe, M., Kojima, S. and Machida, Y. : Formation of a symmetric flat leaf lamina in Arabidopsis Morphogenesis and Pattern Formation in Biological Systems: Experiments and Models (ed. By Sekimura T., Noji, N., Ueno, N. and Maini, P.K.) published by Springer-Verlag 177-187, 2003

Iwakawa H., Ueno .Y, Semiarti E., Onouchi H., Kojima S., Tsukaya H., Hasebe Y., Soma T., Ikezaki M., Machida C., and Machida Y. : The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper. Plant and Cell Physiology 43, 467-478, 2002

Tanaka H., Watanabe M., Watanabe D., Tanaka T., Machida C. and Machida, Y. : 2002, ACR4, a putative receptor kinase gene of Arabidopsis thaliana that is expressed in the outer cell layers of embryos and plants, is involved in proper embryogenesis. Plant and Cell Physiology 43, 419-428, 2002

Tanaka H., Onouchi H., Kondo M., Hara-Nishimura, I., Nishimura M., Machida C. and Machida, Y. : A subtilisin-like serine protease is required for epidermal surface formation in Arabidopsis embryos and juvenile plants. Development 128, 4681-4689, 2001

Semiarti, E., Ueno, Y., Tsukaya, H., Iwakawa H., Machida C. and Machida, Y. : The ASYMMETRIC LEAVES2gene of Arabidopsis thaliana regulates lamina formation, establishment of venation and repression of meristem-related homeobox genes in the leaf. Development 128,1771-1783, 2001

Semiarti E., Onouchi H., Torikai S., Ishikawa T., Machida Y. and Machida C. : The transposition pattern of the Ac element in tobacco cultured cells. Genes and Genetic Systems 76, 131-139, 2001

Nakagawa Y., Machida C., Machida Y. and Toriyama K. : A system to induce deletion of genomic sequences using R/RS site-specific recombination and the Ac transposon in transgenic rice plants. Theoretical and Applied Genetics 102, 1136-1141, 2001

Hamada S., Onouchi H., Tanaka H., Kudo M., Liu, Y.-G., Shibata D., Machida C. and Machida Y. : Mutations in the WUSCHEL gene of Arabidopsis thaliana result in the development of shoot without juvenile leaves. Plant J. 24, 91-103, 2000

Nakagawa Y., Machida C., Machida Y. and Toriyama, K. : Frequency and pattern of transposition of the maize transposable element Ds in transgenic plants. Plant & Cell Physiology 41, 733-742, 2000

Kojima S., Banno H., Yoshioka Y., Oka A., Machida C., and Machida Y. : A binary vector plasmid for gene expression in plant cells that is stably maintained in agrobacterium cells. DNA research 6, 407-410, 1999

Torres M.A., Onouchi H., Hamada S., Machida C., Hammond-Kosack K.E., Jones J.D. : Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox). Plant J. 14, 365-370, 1998

Machida C., Onouchi H., Koizumi J., Hamada S., Semiarti E., Torikai S. and Machida Y. : Characterization of the transposition pattern of the Ac transposable element in Arabidopsis thaliana using endonuclease I-SceI. Proc. Natl. Acad. Sci. USA, 94, 8675-8680, 1997

Machida C. and Machida Y. : Regulation of IS1 transposition by the insA gene product . J. Mol. Biol. 208, 567-574, 1989

Machida C. and Machida Y. : Base substitutions in transposable element IS1 cause DNA duplication of variable length at the target site for plasmid cointegration. The EMBO J. 6, 1799-1803, 1987

Machida C., Machida Y. and Ohtsubo E. : Both inverted repeat sequences of the insertion element IS1 provide promoter function for RNA polymerase. J. Mol. Biol. 177, 247-265, 1984

Machida Y., Machida C. and Ohtsubo E. : The insertion element IS1 encodes two structural genes responsible for its transposition . J. Mol. Biol. 177, 229-245, 1984

Wishart W. L., Machida C., Ohtsubo H. and Ohtsubo E. : E. coli RNA Polymerase binding sites and Transcription initiation sites in the transposon Tn3. Gene 24, 99-113, 1983

Machida C., Machida Y., Wang H.-C. R., Ishizaki K. and Ohtsubo E. : Repression of cointegration ability of insertion element IS1 by transcriptional readthrough from flanking regions. Cell 34, 135-142, 1983

Machida Y., Machida C. and Ohtsubo E. : A novel type of transposon generated by insertion element IS102 present in a pSC101 derivative. Cell 30, 29-36, 1982

Machida Y., Machida C., Ohtsubo H. and Ohtsubo E. : Factors Determining Recombination Frequency Mediated by an Insertion Sequence IS1. Proc. Natl. Acad. Sci. USA 79, 277-281, 1982

Ohtsubo E., Zenilman M., Ohtsubo H., McCormick M., Machida C. and Machida Y.: Mechanism of Insertion and Cointegration Mediated by IS1 and Tn3 Cold Spring Harb. Symp. Quant. Biol. 45, 283-295, 1980

講演、シンポジウム、学会発表

国際会議（2005年以降）

Iwasaki, M., Kojima, S., Matsumura, Y., Ueno, Y., Machida, Y. and Machida, C. 2008.　A genetic screen for genes interacting with AS2 during leaf development. 19th International Conference on Arabidopsis Reseach, at Montreal, Canada

Machida C.: The ASYMMETRIC LEAVES2 and ASYMMETRIC LEAVES1 genes in Arabidopsis regulate the expression of abaxial polarity genes targeted with ta-siRNA in leaves. Molecular Biology Society of Japan, The 7th MBSJ Spring Symposium (at Awajishima), April 23, 2007

Iwakawa H., Iwasaki M., Kojima S., Takahashi H., Kobayashi T., Machida Y. and Machida C., The ASYMMETRIC LEAVES2 gene regulates adaxial cell proliferation involved in the development of symmetric and flat leaf laminae. 18th International Conference on Arabidopsis Reseach, June, 2007 (Beijing, CHN)

Iwasaki M., Iwakawa H., Ueno Y., Takahashi H., Kojima S., Kobayashi T., Machida Y. and Machida C. The ASYMMETRIC LEAVES2 regulates leaf polarity by repressing ETTIN in Arabidopsis. 18th International Conference on Arabidopsis Reseach, June, 2007 (Beijing, CHN)

Semiarti E., Indrianto A., Purwantoro A., Isminingsih S., Suseno N., Ishikawa T., Yoshioka Y., Machida Y. and Machida C. The Use of Arabidopsis BP/KNAT1 gene in Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis 18th International Conference on Arabidopsis Reseach, June, 2007 (Beijing, CHN)

Tanaka H.,Tanaka T., Machida C. and Watanabe M. : Arabidopsis requires signal pathways for the formation of epidermal cuticle. XVII International Botanical Congress Vienna, 17 - 23 July, 2005 (Austria, Europe)

Ishikawa T., Iwakawa H., Ueno Y., Kitakura S., Machida Y. and Machida C. : Subcellular localization of AS1 and AS2 proteins that regulate leaf development in Arabidopsis thaliana.　 The 20th IUBMB Congress and 11th FAOBMB Congress, June, 2006 (Kyoto, Japan)

Kojima S., Matsumura Y.,Ueno Y., Machida Y. and Machida C.: A genetic screen for new factors involved in ASYMMETRIC LEAVES2-mediated leaf developmental pathway. The 20th IUBMB Congress and 11th FAOBMB Congress, June, 2006 (Kyoto, Japan)

Ueno Y., Machida C. and Machida Y.: The distribution of microRNA165/166 in Arabidopsis involves ASYMMETRIC LEAVES2 and histone deacetylase. 17th International Conference on Arabidopsis Reseach, Univ. of Wisconsin, June, 2006 (Madison, USA)

Kojima S., Iwakawa H., Ueno Y., Semiarti E., Soma T., Kuzumaki A.,Tsukaya H., Machida C. and Machida Y. : Function of the ASYMMETRIC LEAVES1 and ASYMMETRIC LEAVES2 genes in leaf development of Arabidopsis. International symposium on PLANT AXIS FORMATION AND SIGNAL TRANSDUCTION, March, 2005 (Tokyo, Japan)

Tanaka H., Watanabe M., Hiroe T., Tsukaya H., Machida C. and Machida Y. : Genetic analysis of genes involved in differentiation of the protoderm and epidermis in Arabidopsis thaliana. International symposium on PLANT AXIS FORMATION AND SIGNAL TRANSDUCTION, March, 2005 (Tokyo , Japan).

Ogasawara F., Ikezaki M., Ueno Y., Machida C. and Machida Y. : Role of class 1 knox genes in asymmetric leaves1 mutant in Arabidopsis thaliana 16th International Conference on Arabidopsis Reseach, Univ. of Wisconsin, June, 2005 (Madison, USA)

Kojima S., Ueno Y., Iwakawa H., Semiarti E., Soma T., Tsukaya H., Ishikawa T., Machida Y. and Machida C. : ”The ASYMMETRIC LEAVES2 is involved in the formation of a flat leaf lamina in the presence of the ASYMMETRIC LEAVES1 gene”, 16th International Conference on Arabidopsis Reseach, Univ. of Wisconsin, June, 2005 (Madison, USA)

受賞

The 80th Annual Meeting of Genetics Society of Japan Best Paper Award 2008 Machida C., Iwakawa H., Takahashi H., Mazet Rémi, Iwasaki M., Kojima S., Kobayashi T. and Machida Y. : Analysis of genetic network of gene regulation mediated by AS2 and AS1 in the leaf development in Arabidopsis thaliana September 2009 (Nagoya)

Nagoya International Orchid Congress（NIOC）Semiarti, E., Indrianto, A., Purwantoro, A., Isminingsih, S., Suseno, N., Machida, Y., Kojima, S. and Machida, C.：Development of methods for Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis and its application. Nagoya International Orchid Congress 2009 (Nagoya)

社会活動

日本植物学会（学会賞選考委員）2009-2010

日本植物学会（評議委員）2005-2008

日本植物生理学会（運営委員）2003-2004

日本植物生理学会（評議委員）2002-2005

Plant and cell physiology（編集委員）2002-2005

その他

特許

国際出願：PCT/JP2008/056227 町田泰則、エンダン・セミアルティ、町田千代子 ラン科植物の形質転換方法 国際出願日：2008年3月28日

特願2007-095868 ラン科植物の形質転換方法 町田泰則、エンダン・セミアルティ、町田千代子

研究費採択状況

科学研究費補助金

2009-2011　基盤研究C 研究代表者

2007-2012 特定領域研究　植物メリステム 計画研究 (研究分担者)

2007-2008 基盤研究C 研究代表者

2003-2004 基盤研究C 研究代表者

2001-2005 特定領域研究　発生システムのダイナミクス 公募研究　(研究代表者)

科学研究費補助金以外の研究費

2005-2009 私立大学学術研究高度化推進事業学術フロンティア推進事業（文部科学省）(研究代表者)

2001-2006 CREST(独立行政法人科学技術振興機構)（代表　岡田清孝）(研究分担者)

その他の科学研究費補助金

2008 ひらめき☆ときめき サイエンス〜ようこそ大学の研究室へ〜KAKENHI (研究代表者)