CHUBU UNIVERSITY
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OHTSUKA Kenzo

Profile

Year of Birth 1950
Place of Birth Akita Prefecture
Title Professor
Belong to Dept. of Environmental Biology
Bioscience and Biotechnology (graduate school)
Radioisotope Research Center
Graduated Osaka University, Graduate School of Science
Degree Dr. of Science, Osaka University
Academic Institutional Membership Japanese Society for Cell Biology, The Japanese Biochemical Society,
The Molecular Biology Society of Japan,
The Japanese Cancer Association,
The Japan Radiation Research Society,
Asian Society of Hyperthermia Oncology, Society for Thermal Medicine,
The Biomedical Society for Stress Response
Field of Study Cell Biology, Molecular Biology, Cell and Stress Biology
Research, Studies Environmental Stress Response, Structures and Functions of Heat Shock Proteins and Molecular Chaperones
Curriculum Specialized Animal Physiology, Cell Biology

Books Published, Translation

Hyperthermic Oncology from Bench to Bedside (joint work), Springer 2016

Thermotherapy for Neoplasia, Inflammation and Pain (joint work), Springer-Verlag, 2000

Guidebook to Molecular Chaperones and Protein Folding Catalysts (joint work), Oxford University Press, 1997

Academic Papers, Critique

078. Ihara M, Shichijo K, Kudo T, Otsuka K. Reactivation of heat-inactivated Ku proteins by heat shock cognate protein HSC73. Int J Hyperthermia 36: 438-443, 2019.

077. Tohnai G, Adachi H, Katsuno M, Doi H, Matsumoto S, Kondo N, Miyazaki Y, Iida M, Nakatsuji H, Qiang Q, Ding Y, Watanabe H, Yamamoto M, Ohtsuka K, Sobue G.: Paeoniflorin eliminates a mutant AR via F-YA-dependent proteolysis in spinal and bulbar muscular atrophy. Hum. Mol. Genet. 23: 3552-3565, 2014.

076. Tabuchi Y, Furusawa Y, Kariya A, Wada S, Ohtsuka K, Kondo T.: Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells. Int. J. Hyperthermia. 29:38-50, 2013.

075. Hisawa, T., Utsumi, T., Yasuraoka, M., Hanamura N., Shimada, H., Nakajima, H., Kitagawa, M., Iwadate, Y., Goto, K., Takeda, A., Ohtsuka, K., Ariga, H. and Takiguchi, M.: Functional similarity of anticancer drugs by MTT bioassay. J. Cancer Science and Therapy, 3: 250-255, 2011.

074. Tabuchi, Y., Wada, S., Furusawa, Y., Ohtsuka K. and Kondo, T.: Gene networks related to the cell death elicited by hyperthermia in human oral squamous cell carcinoma HSC-3 cells. Int. J. Mol. Med. 29: 380-386, 2012.

073. Tabuchi, Y., Furusawa, Y., Wada, S., Ohtsuka, K. and Kondo, T.: Silencing heat shock transcription factor I using small interfering RNA enhances mild hyperthermia and hyperthermia sensitivity in human oral squamous cell carcinoma cells. Thermal Medicine, 27: 99-108, 2011.

072. Hisawa, T., Utsumi, T., Yasuraoka, M., Hanamura N., Shimada, H., Nakajima, H., Kitagawa, M., Iwadate, Y., Goto, K., Takeda, A., Ohtsuka, K., Ariga, H. and Takiguchi, M.: Functional similarity of anticancer drugs by MTT bioassay. J. Cancer Science and Therapy, 3: 250-255, 2011.

072. Furusawa, Y., Tabuchi, Y., Wada, S., Takasaki, I., Ohtsuka, K. and Kondo, T.: Identification of biological functions and gene networks regulated by heat stress in U937 human lymphoma cells. Int. J. Mol. Med., 28: 143-151, 2011.

071. Takeuchi, R., Soga, M., Inaguma, Y. and Ohtsuka, K.: Characterization of a novel mammalian co-chaperone, DnaJB7, an HSP40 family member. Thermal Medicine, 27: 9-23, 2011.

070. Asai, M., Kawashima D., Katagiri K., Takeuchi R., Tohnai G. and Ohtsuka, K.: Protective effect of a molecular chaperone inducer, paeoniflorin, on the HCl- and ethanol-triggered gastric mucosal injury. Life Sciences, 88: 350-357, 2011.

069. Kawashima, D., Soga, M., Takeuchi, R., Matsumoto, H. and Ohtsuka, K.: Molecular chaperone inducers facilitate the functional restoration of temperature-sensitive mutant p53 protein. Thermal Medicine 26: 1-17, 2010.

068. Furusawa, Y., Tabuchi, Y., Takasaki, I., Wada, S., Ohtsuka, K. and Kondo, T.: Gene networks involved in apoptosis induced by hyperthermia in human lymphoma U937 cells. Cell Biol. Int. 33: 1253-1262, 2009.

067. Kawashima. D., Asai, M., Katagiri, K., Takeuchi, R. and Ohtsuka, K.: Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins. Cell Stress & Chaperones 14:535-543, 2009.

066. Tabuchi, Y., Takasaki, I., Wada, S., Zhao, Q-L., Hori, T., Nomura, T., Ohtsuka, K. and Kondo, T.: Genes and genetic networks responsive to mild hyperthermia in human lymphoma U937 cells. Int. J. Hyperthermia 24: 613-622, 2008.

065. Ohtsuka, K., Kawashima, D. and Asai, M: Dual functions of heat shock proteins: Molecular chaperones inside of cells and danger signals outside of cells. Thermal Medicine 23: 11-22, 2007. (Review)

064. Salunga, T.L., Tabuchi, Y., Takasaki, I., Feril Jr., L.B., Zhao, Q-L., Ohtsuka, K., Tsuneyama, K. and Kondo, T.: Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells. Int. J. Hyperthermia 23: 529-537, 2007.

063. Tabuchi, Y., Kuribayashi, R., Takasaki, I., Doi, T., Sakai, H., Takeguchi, N., Kondo, T. and Ohtsuka, K.: Overexpression of Hsp70 restores the structural stability and functional defects of temperature-sensitive mutant of large T-antigen at nonpermissive temperature. Cell Stress & Chaperones 11: 259-267, 2006.

062. Ohtsuka, K., Kawashima D., Gu, Y. and Saito, K.: Inducers and co-inducers of molecular chaperones.Int. J. Hyperthermia 21: 703-711, 2005. (Review)

061. Saito, K., Dai, Y. and Ohtsuka, K.: Enhanced expression of heat shock proteins in gradually dying cells and their release from necrotically dead cells. Exptl. Cell Res. 310: 229-236, 2005.

060. Nozawa, N., Yamauchi, Y., Ohtsuka, K., Kawaguchi, Y. and Nishiyama, Y.: Formation of aggresome-like structures in herpes simplex virus type 2-infected cells and a potential role in virus assembly. Exptl. Cell Res. 299: 486-497, 2004.

059. Dai, Y., Saito, K., Ohmi, Y., Fujie, N. and Ohtsuka, K.: Paeoniflorin, a novel heat shock protein-inducing compound. Cell Stress & Chaperones 9: 378-389, 2004.

058. Asai, T., Takahashi, T., Esaki, M., Nishikawa, S., Ohtsuka, K., Nakai, M. and Endo, T.: Reinvestigation of the requirement of cytosolic ATP for mitochondrial protein import. J. Biol. Chem. 279: 19464-19471, 2004.

057. Sato, S., Oka, M., Noguchi, Y., Soda, H., Tsurutani, J., Nakamura, Y., Kitazaki, T., Mizuta, Y., Takeshima, F., Murase, K., Murata, I., Ohtsuka, K. and Kohno S.: Autoimmunity to heat shock protein 40 in ulcerative colitis. J. Int. Med. Res. 32: 141-148, 2004.

056. Liu, Q-L., Kishi, H., Ohtsuka, K. and Muraguchi, A.: Heat-shock protein 70 binds caspase-activated DNase and enhances its activity in TCR-stimulated T cells. Blood 102: 1788-1796, 2003.

055. Isomoto, H., Oka, M., Yano, Y., Kanazawa, Y., Soda, H., Terada, R., Yasutake, T., Nakayama, T., Shikuwa, S., Takeshima, F., Udono, H., Murata, I., Ohtsuka, K. and Kohno, S.: Expression of heat shock protein (Hsp) 70 and Hsp40 in gastric cancer. Cancer Lett. 198: 219-226, 2003.

054. Kanazawa, Y., Isomoto, H., Oka, M., Yano, Y., Soda, H., Shikuwa, S., Takeshima, F., Omagari, K., Mizuta, Y., Murase, K., Nakagoe, T., Ohtsuka, K. and Kohno, S.: Expression of heat shock protein (Hsp) 70 and Hsp40 in colorectal cancer. Medical Oncol. 20: 157-164, 2003.

053. Ishihara, K., Yamagishi, N., Saito, Y., Adachi, H., Kobayashi, Y., Sobue, G., Ohtsuka, K., Hatayama, T.: Hsp105alpha suppresses the aggregation of truncated androgen receptor with expanded CAG repeats and cell toxicity. J. Biol. Chem. 278: 25143-25150, 2003.

052. Murata, T., Goshima, F., Nishizawa, Y., Daikoku, T., Takakuwa, H., Ohtsuka, K., Yoshikawa, T. and Nishiyama, Y.: Phosphorylation of cytoskeleton 17 by Herpes simplex virus type 2 US3 protein kinase. Microbiol. Immunol. 46: 707-719, 2002.

051. Yamauchi Y, Wada K, Goshima F, Daikoku T, Ohtsuka K, Nishiyama Y.: Herpes simplex virus type 2 UL14 gene product has heat shock protein (HSP)-like functions. J. Cell Sci. 115: 2517-2527, 2002.

050. Takeuchi, H., Kobayashi, Y., Yoshihara, T., Niwa, J., Doyu, M., Ohtsuka, K. and Sobue, G.: Hsp70 and Hsp40 improve neurite outgrowth and suppress intracytoplasmic aggregate formation in cultured neuronal cells expressing mutant SOD1. Brain Res. 949: 11-22, 2002.

049. Hata, M., Kagotani, K., Okumura, K., Seto, M. and Ohtsuka, K.: Characterization of a processed pseudogene of human ΨHSP40 on chromosome 2q32. DNA Seq. 12: 293-297, 2001.

048. Oka, M., Sato, S., Soda, H., Fukuda, M., Kawabata, S., Nakatomi, K., Shiozawa, K., Nakamura, Y., Ohtsuka, K. and Kohno, S.: Autoantibody to heat shock protein Hsp40 of lung cancer patients. Jpn. J. Cancer Res. 92: 316-320, 2001.

047. Ohtsuka, K. and Hata, M.: Molecular chaperone function of mammalian Hsp70 and Hsp40 - A review. Int. J. Hyperthermia 16: 231-245, 2000.

046. Ohtsuka, K. and Suzuki, T.: Roles of molecular chaperones in the nervous system. Brain Res. Bull. 53: 141-146, 2000. (Review)

045. Izawa, I., Nishizawa, M. Ohtakara, K., Ohtsuka, K., Inada, H. and Inagaki, M.: Identification of Mrj, a DnaJ/Heat shock protein 40 family protein, as a keratin 8/18 filament-regulatory protein. J. Biol. Chem. 275(44): 34521-34527, 2000.

044. Yokoyama, N., Hirata, M., Ohtsuka, K., Fujii, K., Fujita, M., Kuzushima, K., Kiyono, T. and Tsurumi, T.: Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor, increases solubility of overexpressed target proteins in insect cells. Biochim. Biophys. Acta 1493(1-2): 119-124, 2000.

043. Hata, M. and Ohtsuka, K.: Murine cDNA encoding a novel type I HSP40/DNAJ homolog, mmDjA4. Biochim. Biophys. Acta 1493(1-2): 208-210, 2000.

042. Hata, M. and Ohtsuka, K.: Cloning and expression of murine Hsp40 gene: Differences in initiation sites between heat-induced and constitutive transcript. DNA Seq. 11: 213-223, 2000.

041. Yamagishi, N., Nishihori , H., Ishihara, K., Ohtsuka, K. and Hatayama, T.: Modulation of the chaperone activities of Hsc70/Hsp40 by Hsp105a and Hsp105b. Biochem. Biophys. Res. Commun. 272(3): 850-855, 2000.

040. Kobayashi, Y., Kume, A., Li, M., Doyu, M., Hata, M., Ohtsuka, K. and Sobue, G.: Chaperones Hsp70 and Hsp40 suppress aggregate formation and apoptosis in cultured neuronal cells expressing truncated androgen receptor protein with expanded polyglutamine tract. J. Biol. Chem. 275: 8772-8778, 2000.

039. Ohtsuka, K. and Hata, M.: Mammalian HSP40/DNAJ homologs: Cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress & Chaperones 5: 98-112, 2000.

038. Mori, T., Sugito, K., Hata, M., Tohnai, I., Hayashi, Y., Ueda, M. and Ohtsuka, K.: Mammalian Hsp70 and Hsp40: Characteristic induction by environmental stresses and tissue specific expression. Jpn. J. Hyperthermic Oncol. 15: 21-29, 1999.

037. Suziki, T., Usuda, N., Murata, S, Nakazawa, A., Ohtsuka, K. and Takagi, H.: Presence of molecular chaperones, heat shock cognate (Hsc) 70 and heat shock proteins (Hsp) 40, in the postsynaptic structures of rat brain. Brain Res. 816: 99-110, 1999.

036. Hata, M. and Ohtsuka, K.: Characterization of HSE sequences in human hsp40 gene: Structural and promoter analysis. Biochim. Biophys. Acta 1397: 43-55, 1998.

035. Michels, A.A., Kanon, B., Konings, A.W.T., Ohtsuka, K., Bensaude, O. and Kampinga, H.H.: Hsp70 and Hsp40 chaperone activities in the cytoplasm and the nucleus of mammalian cells. J. Biol. Chem. 272: 33283-33289, 1997.

034. Kaneko, R., Hayashi, Y., Tohnai, I., Ueda, M. and Ohtsuka, K.: Hsp40, a possible indicator for thermotolerance of murine tumor in vivo. Int. J. Hyperthermia 13: 507-516, 1997.

033. Terada, K., Ueda, I., Ohtsuka, K., Oda, T., Ichiyama, A. and Mori, M.: The requirement of heat shock cognate 70 protein for mitochondrial import varies among precursor proteins and depending on precursor length. Mol. Cell. Biol. 16: 6103-6109, 1996.

032. Hata, M., Okumura, K., Seto, M. and Ohtsuka, K.: Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2. Genomics 38: 446-449, 1996.

031. Minami, Y., Hohfeld, J., Ohtsuka, K. and Hartl, F.U.: Regulation of the heat-shock protein 70 reaction cycle by the mammalian DnaJ homolog, Hsp40. J. Biol. Chem. 271: 19617-19624, 1996.

030. Yamane, M., Sugito, K., Hattori, H., Hayashi, Y., Tohnai, I., Ueda, M., Nishizawa, K. and Ohtsuka, K.: Cotranslocation and colocalization of hsp40 (DnaJ) with hsp70 (DnaK) in mammalian cells. Cell Struct. Funct. 20: 157-166, 1995.

029. Terada, K., Ohtsuka, K., Imamoto, N., Yoneda, Y. and M. Mori.: Role of heat shock cognate 70 protein in import of ornithine transcarbamylase precursor into mammalian mitochondria. Mol. Cell. Biol. 15, 3708-3713, 1995.

028. Kaneko, R., Hattori, H., Hayashi, Y., Tohnai, I., Ueda, M. and Ohtsuka, K.: Heat-shock protein 40, a novel predictor of thermotolerance in murine cells. Radiat. Res. 142: 91-97, 1995.

027. Sugito, K., Yamane, M., Hattori, H., Hayashi, Y., Tohnai, I., Ueda, M., Tsuchida, N. and Ohtsuka, K.: Interaction between hsp70 and hsp40, eukaryotic homologues of DnaK and DnaJ, in human cells expressing mutant-type p53. FEBS Lett. 358: 161-164, 1995.

026. Frydman, J., Nimmesgern, E., Ohtsuka, K. and Hartl, F.U.: Folding of nascent polypeptide chains in a high molecular mass assembly with molecular chaperones. Nature 370: 111-117, 1994.

025. Ohtsuka, K.: Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ. Biochem. Biophys. Res. Commun. 197: 235-240, 1993.

024. Ohtsuka, K., Utsumi, K.R., Kaneda, T. and Hattori, H.: Effect of ATP on the release of hsp 70 and hsp 40 from the nucleus in heat-shocked HeLa cells. Exptl. Cell Res. 209: 357-366, 1993.

023. Mitsudomi, T., Kido, Y., Ohtsuka, K. and Sugimachi, K.: Transformation by viral oncogenes increased sensitivity to heat in rat 3Y1 fibroblasts. Anticancer Res. 13: 995-1000, 1993.

022. Hattori, H., Kaneda, T., Lokeshwar, B., Laszlo, A. and Ohtsuka, K.: A stress-inducible 40-kDa protein (hsp40): Purification by modified two-dimensional gel electrophoresis and co-localization with hsc70(p73) in heat-shocked HeLa cells. J. Cell Sci. 104: 629-638, 1993.

021. Ohtsuka, K., Liu, Y.-C. and Kaneda, T.: Cytoskeletal thermotolerance in NRK cells. Int. J. Hyperthermia 9: 115-124, 1993.

020. Ohtsuka, K. and Laszlo, A.: The relationship between hsp70 localization and heat resistance. Exptl. Cell Res. 202: 507-518, 1992.

019. Liu, Y.-C., Hayashi, Y., Tohnai, I., Kaneda, T. and Ohtsuka, K.: Effects of continuous heating at mild temperatures on the translocation of hsp70 and protein synthesis in NRK cells. J. Radiat. Res. 33: 199-210, 1992.

018. Hattori, H., Liu, Y.-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K. and Ohtsuka, K.: Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells. Cell Struct. Funct. 17: 77-86, 1992.

017. Sakakibara, Y., Shimada, Y., Masuda, A. and Ohtsuka, K.: Development of thermotolerance in hsp70 induction-defective mutant of NRK cells. Int. J. Hyperthermia 8: 329-340, 1992.

016. Hatayama, T., Taniguchi, Y., Kano, E., Furuya, M., Hayashi, S., Ohtsuka, K., Wakatsuki, T., Kitamura, T. and Imahara, H.: Characteristic synthesis and redistribution of 70-kD heat shock protein in thermotolerant Chinese hanster V79 cells. Int. J. Hyperthermia 8: 121-130, 1992.

015. Hayashi, Y., Tohnai, I., Kaneda, T., Kobayashi, T. and Ohtsuka, K.: Translocation of hsp70 and protein synthesis during continuous heating at mild temperatures in HeLa cells. Radiat. Res. 125: 80-88, 1991.

014. Yagi, K., Ishida, N. and Ohtsuka, K.: Induction by chinoform-ferric chelate of lipid peroxidation in rat liver microsomes. J. Clin. Biochem. Nutr. 9: 11-15, 1990.

013. Masuda, A., Ohtsuka, K. and Matsuyama, M.: Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus. In Vitro Cell. & Dev. Biol. 26: 713-721, 1990.

012. Nakai, A., Hirayama, C., Ohtsuka, K., Hirayoshi, K. and Nagata, K.: Novel ATP-binding heat inducuble protein of Mr=37,000 that is sensitive to transformation in BALB/3T3 cells. J. Cell. Physiol. 143: 577-589, 1990.

011. Ohtsuka, K., Masuda, A., Nakai, A. and Nagata, K.: A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells. Biochem. Biophys. Res. Commun. 166: 642-647, 1990.

010. Sato, C., Nishizawa, K., Nakayama, T., Ohtsuka, K., Nakamura, H., Kobayashi, T. and Inagaki, M.: Rapid phosphorylation of MAP-2-related cytoplasmic and nuclear Mr 300,000 protein by serine kinases after growth stimulation in quiescent cells. Exptl. Cell Res. 175: 136-147, 1988.

009. Ohtsuka, K., Tanabe, K., Nakamura, H. and Sato, C.: Possible cytoskeletal association of 69,000 and 68,000 dalton heat shock proteins and structural relations among heat shock proteins in murine mastocytoma cells. Radiat. Res. 108: 34-42, 1986.

008. Ohtsuka, K., Furuya, M. Nitta, K. and Kano, E.: Effect of cycloheximide on the development of thermotolerance and the synthesis of 68-kilodalton heat shock protein in Chinese hamster V79 and mouse L cells in vitro. J. Radiat. Res. 27: 291-299, 1986.

007. Ohtsuka, K., Nakamura, H. and Sato, C.: Intracellular distribution of 73,000 and 72,000 dalton heat shock proteins in HeLa cells. Int. J. Hyperthermia 2: 267-275, 1986.

006. Ohtsuka, K. and Nakamura, W.: Modification of the thermal sensitivity of the murine foot and tumor by prior hypoxic treatment. Int. J. Hyperthermia 2: 65-73, 1986.

005. Ohtsuka, K. and Nakamura, W.: Induction of thermotolerance in mouse foot with prior hypoxic treatment. J. Radiat. Res. 27: 219-224, 1986.

004. Yagi, K., Ohtsuka, K. and Ohishi, N.: Lipid peroxidation caused by chinoform-ferric chelate in cultured neural retinal cells. Experientia 41: 1561-1563, 1985.

003. Ohtsuka, K. Ohishi, N. Eguchi, G. and Yagi, K.: Degeneration of retinal neuroblasts by chinoform-ferric chelate. Experientia 38: 120-122, 1982.

002. Ohtsuka, K. and Tonomura, Y.:Characterization and partial purification of the factor(s) stimulating the beating of cultured myocardial cells from a conditioned medium. Cell Struct. Funct. 3: 1-9, 1978.

001. Ohtsuka, K. and Tonomura, Y.: Effects of conditioning of medium on beating of single myocardial cells in vitro. Cell Struct. Funct. 1: 11-21, 1975.

Awards

JSTM (Japanese Society for Thermal Medicine) Award, Sept. 24, 2004.

JSTM (Japanese Society for Thermal Medicine) Young Investigator Award, Dec. 25, 1985.

Social Activities

Councilor of the Japanese Society of Hyperthermic Oncology

Councilor of the Biomedical Society for Stress Response

Editorial Board (International Journal of Hyperthermia)

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